以下是一个示例代码，演示如何使用Amazon S3传输管理器进行目录上传，并计算哈希值。

import os
import hashlib
import boto3
from botocore.exceptions import NoCredentialsError

def upload_directory_to_s3(local_directory, bucket, s3_folder):
    s3 = boto3.client('s3')
    for root, dirs, files in os.walk(local_directory):
        for file in files:
            local_path = os.path.join(root, file)
            relative_path = os.path.relpath(local_path, local_directory)
            s3_path = os.path.join(s3_folder, relative_path).replace("\\", "/")
            try:
                s3.upload_file(local_path, bucket, s3_path)
                print("Successfully uploaded file to S3: " + s3_path)
            except FileNotFoundError:
                print("The file was not found")
            except NoCredentialsError:
                print("Credentials not available")

def calculate_hash(file_path):
    with open(file_path, 'rb') as f:
        hasher = hashlib.sha256()
        buf = f.read()
        while len(buf) > 0:
            hasher.update(buf)
            buf = f.read()
        return hasher.hexdigest()

# 设置本地目录，S3存储桶和S3文件夹路径
local_directory = "/path/to/local/directory"
bucket = "your-s3-bucket"
s3_folder = "your-s3-folder"

# 上传目录到S3
upload_directory_to_s3(local_directory, bucket, s3_folder)

# 计算目录中每个文件的哈希值
for root, dirs, files in os.walk(local_directory):
    for file in files:
        local_path = os.path.join(root, file)
        relative_path = os.path.relpath(local_path, local_directory)
        s3_path = os.path.join(s3_folder, relative_path).replace("\\", "/")
        s3 = boto3.resource('s3')
        obj = s3.Object(bucket, s3_path)
        print("File: " + s3_path + ", Hash: " + calculate_hash(local_path))

请确保安装了必要的Python库，如boto3和botocore。 另外，还需要配置好AWS凭证，以便进行上传操作。